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Fixed sections were washed in PBS, permeabilized with 0.3% Nonidet P-40 for 5 min, and blocked with 2.5% normal goat serum (NGS) for 1 hour

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Semaglutide entered the diabetes market in 2017 and showed such remarkable weight loss as a side effect that researchers conducted dedicated obesity trials—leading to its FDA approval for chronic weight management in 2021

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